Volume 7.16 | Apr 24

Neural Cell News 7.16 April 24, 2013
     In this issue: Publications | Reviews | Industry News | Policy News | Events | Jobs
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TOP STORY
New Use for Stem Cells Identifies a Promising Way to Target ALS
Using a new, stem cell-based, drug-screening technology that could reinvent and greatly reduce the cost of developing pharmaceuticals, researchers at the Harvard Stem Cell Institute have found a compound that is more effective in protecting the neurons killed in amyotrophic lateral sclerosis (ALS) than are two drugs that failed in human clinical trials after large sums were invested in them. [Press release from Harvard Stem Cell Institute discussing online prepublication in Cell Stem Cell] Press Release | Abstract | Graphical Abstract

Webinar: Brent Reynolds on Identification and Characterization of Neural Stem Cells
PUBLICATIONS (Ranked by impact factor of the journal)

Medial Ganglionic Eminence-Like Cells Derived from Human Embryonic Stem Cells Correct Learning and Memory Deficits
The authors present a method for differentiating human embryonic stem cells to a nearly uniform population of NKX2.1+ medial ganglionic eminence (MGE)-like progenitor cells. Mice transplanted with MGE-like but not spinal progenitors showed improvements in learning and memory deficits. [Nat Biotechnol]
Abstract | Press Release

Peli1 Promotes Microglia-Mediated CNS Inflammation by Regulating Traf3 Degradation
Researchers showed that the E3 ubiquitin ligase Peli1 is abundantly expressed in microglia and promotes microglial activation during the course of experimental autoimmune encephalomyelitis induction. Peli1 mediates the induction of chemokines and proinflammatory cytokines in microglia and thereby promotes recruitment of T cells into the central nervous system (CNS). [Nat Med] Abstract

Subcellular Localization Determines the Stability and Axon Protective Capacity of Axon Survival Factor Nmnat2
Researchers investigated the mechanism of fast axonal transport of Nicotinamide mononucleotide adenlylyltransferase (Nmnat2) and its site of action for axon maintenance. Using dual-color live-cell imaging of axonal transport in superior cervical ganglion primary culture neurons, they found that Nmnat2 is bidirectionally trafficked in axons together with markers of the trans-Golgi network and synaptic vesicles. [PLoS Biol] Full Article

Autocrine Signaling Based Selection of Combinatorial Antibodies that Transdifferentiate Human Stem Cells
Researchers report the generation of antibody agonists from intracellular combinatorial libraries that transdifferentiate human stem cells. Unlike the natural granulocyte-colony stimulating factor that activates cells to differentiate along a predetermined pathway, the isolated agonist antibodies transdifferentiated human myeloid lineage CD34+ bone marrow cells into neural progenitors. [Proc Natl Acad Sci USA]
Abstract | Press Release


TFEB-Mediated Autophagy Rescues Midbrain Dopamine Neurons from α-Synuclein Toxicity
Using an adeno-associated virus model of Parkinson disease to overexpress α-synuclein in the substantia nigra, researchers showed that genetic transcription factor EB (TFEB) and Beclin-1 overexpression or pharmacological manipulations that enhance autophagy protect nigral neurons from α-synuclein toxicity, but inhibiting autophagy exacerbates α-synuclein toxicity. [Proc Natl Acad Sci USA] Abstract


The Therapeutic Activities of Engrafted Neural Stem/Precursor Cells are Not Dormant in the Chronically Injured Spinal Cord
Researchers performed temporal analysis of injured spinal cords and demonstrated their multiphasic cellular and molecular responses. In particular, chronically injured spinal cords were growth factor-enriched environments, whereas acutely injured spinal cords were enriched by neurotrophic and inflammatory factors. To determine how these environmental differences affect engrafted cells, neural stem/precursor cells transplanted into acutely, subacutely, and chronically injured spinal cords were selectively isolated by flow-cytometry, and their whole transcriptomes were compared by RNA-sequencing. [Stem Cells] Abstract

Doxycycline Restrains Glia and Confers Neuroprotection in a 6-OHDA Parkinson Model
Results suggest that doxycycline blocks 6-OHDA neurotoxicity in vivo by inhibiting microglial and astrocyte expression. This action of doxycycline in nigrostriatal dopaminergic neuron protection is consistent with a role of glial cells in Parkinson’s disease neurodegeneration. [Glia] Abstract

The LIM Homeobox Gene ceh-14 Is Required for Phasmid Function and Neurite Outgrowth
The authors previously showed that the LIM homeodomain factor CEH-14 is expressed in the AFD neurons where it is required for thermotaxis behavior in Caenorhabditis elegans. In this study, they showed that ceh-14 is expressed in the phasmid sensory neurons, PHA and PHB, a number of neurons in the tail, i.e., PHC, DVC, PVC, PVN, PVQ, PVT, PVW and PVR, as well as the touch neurons. [Dev Biol] Abstract

Morphological and Functional Differentiation in BE(2)-M17 Human Neuroblastoma Cells by Treatment with Trans-Retinoic Acid
The clonal human neuroblastoma BE(2)-M17 cell line is known to differentiate into a more prominent neuronal cell type by treatment with trans-retinoic acid. Researchers studied the effects of trans-retinoic acid treatment on (a) some differentiation marker proteins, (b) types of voltage-gated calcium (Ca2+) channels and (c) Ca2+-dependent neurotransmitter ([3H] glycine) release in cultured BE(2)-M17 cells. [BMC Neurosci]
Abstract | Full Article


Expression of the Protein Chaperone, Clusterin, in Spinal Cord Cells Constitutively and Following Cellular Stress, and Upregulation by Treatment with Hsp90 Inhibitor
To assess relevance to amyotrophic lateral sclerosis (ALS) and other motor neuron disorders, clusterin expression was evaluated using long-term dissociated cultures of murine spinal cord and SOD1G93A transgenic mice, a model of familial ALS. Motor neurons and astrocytes constitutively expressed nuclear and cytoplasmic forms of clusterin, and secreted clusterin accumulated in culture media. [Cell Stress Chaperones] Abstract

Gain Hands-On Training - New Course: Differentiation of hPSCs to Neural Progenitor Cells

REVIEWS

Progress in Gene Therapy for Neurological Disorders
The authors describe the promising gene therapy strategies that have the potential to treat patients with neurological diseases and discuss prospects for future development of gene therapy. [Nat Rev Neurol] Abstract

Vesicle Dynamics: How Synaptic Proteins Regulate Different Modes of Neurotransmission
The authors evaluate recent advances in understanding the regulatory mechanisms of synaptic vesicle dynamics and in understanding how different molecular substrates maintain selective modes of neurotransmission. They also highlight the implications of these studies in understanding pathological conditions. [J Neurochem] Full Article

The Peripheral Neuronal Phenotype Is Important in the Pathogenesis of Painful Human Tendinopathy: A Systematic Review
The primary purpose of this review was to determine whether evidence exists of changes in the peripheral neuronal phenotype in painful human tendinopathy and, if so, to identify the associated histological and molecular changes. The secondary purpose was to determine if any changes in the peripheral neuronal phenotype reported correlate with pain symptoms. [Clin Orthop Relat Res] Abstract

Visit our reviews page to see a complete list of reviews in the neuroscience field.

INDUSTRY NEWS

Ceregene Reports Data from Parkinson’s Disease Phase IIb Study
Ceregene, Inc. announced the top-line data from its double-blind, randomized, controlled Phase IIb clinical study of CERE-120, a gene therapy product designed to deliver the neurotrophic factor neurturin, for Parkinson’s disease. [Ceregene, Inc.] Press Release


Multi-Million Dollar Gift Establishes Brain Tumor Center at Methodist
Brain and pituitary tumor patients will soon have access to a state-of-the-art facility at The Methodist Hospital, a result of a $10 million dollar commitment from a Houston oil executive. [The Methodist Hospital System] Press Release


Neuralstem and the National Football League Alumni Association Announce Initiative on Brain Injury Treatment
Neuralstem, Inc. announced that it is working with the National Football League Alumni Association (NFLAA), based in Newark, NJ, to develop a trial for treating NFL alumni members suffering from traumatic brain injuries with NSI-189, the lead compound in the company’s neurogenic drug platform. [Neuralstem, Inc.] Press Release

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POLICY NEWS

National Institutes of Health (United States)

Food and Drug Administration (United States)

Center for Biologics Evaluation and Research (United States)

European Medicines Agency (European Union)

Medicines and Healthcare Products Regulatory Agency (United Kingdom)

Therapeutic Goods Administration (Australia)

EVENTS
NEW Neurizons 2013 “Solving the Brain Puzzle: Building Minds from Molecules”
May 22-25, 2013
Göttingen, Germany

Visit our events page to see a complete list of events in the neural cell community.
JOB OPPORTUNITIES

Research Associate  – Neuroscience (University College London)

Postdoctoral Researcher – Neuroscience (Copenhagen University)


Postdoctoral Position – Neurobiology (Weizmann Institute of Science)

Postdoctoral Position – Adult Neurogenesis and Neural Survival Signaling (University of Kentucky)

PhD Opportunity – Role of Fibroblast Growth Factors in the Generation of Neuronal Diversity in the Spinal Cord (University of East Anglia)

Doctoral Studentship – Neural Cell Physiology (CNR-ISMN)

PhD Studentship – Decoding the Genetics of Postembryonic Neural Stem Cell Activation (Plymouth University)


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